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Old February 1st, 2018, 10:21 AM
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Default Re: Wbutech Question Papers

I am providing you the question paper of Molecular Genetics of B.Sc Microbiology, Genetics of Maulana Abul Kalam Azad University of Technology

WBUT B.Sc Microbiology, Genetics Molecular Genetics question paper

Time Allotted
: 3 Hours Full Marks : 70

The figures in the margin indicate full marks.

Candidates are required to give their answers in their own words

as far as practicable.


( Multiple Choice Type Questions )

1. Choose the correct alternatives for any ten of the following :

[Marks 10x1 = 10]

i) Which of the following methods is most useful for

enzymatic amplification of specific segment of DNA ?

a) Nucleotide sequencing

b) DNA hybridization

c) PCR

d) None of these.

ii) Gene expression is analyzed by

a) Southern blot b) Restriction digestion

c) Northern blot d) None of these.

iii) In sourthern blotting experiment, the binding of

transferred DNA to the nylon membrane is ……………


a) ionic b) covalent

c) hydrophobic d) van der Waals.

iv) DNA hybridization is a technique, which relies on the

following properties of DNA, except

a) Double strandedness

b) sequence specificity

c) major and minor grooves

d) denaturation-renaturation properties.

v) For RNA detection which PCR is best ?

a) Nested PCR b) RT-PCR


vi) In Western blot the protein samples are run on

a) Agarose gel

b) polyacrylamide gel

c) formaldehyde-agarose gel

d) none of these.

vii) The stringency of a hybridization reaction depends on

all of the following, except

a) NaCl concentration

b) type of reporter molecule

c) nucleotide sequence of probe

d) pH.

viii) Ligase joins two DNA molecules together by forming a

covalent bond between

a) two PO4

b) two OH

c) one PO4 and one OH

d) Complementary nucleotides on opposite strands.

ix) -lactum ring is present in

a) Tetracycline b) Ampicillin

c) Kanamycin d) Streptomycin.

x) Principal function of reporter molecule in DNA

hybridization assay

a) enhance the stringency of hybridization reaction

b) aid in base pairing

c) aid in the detection of probe target hybridization

d) bind the target DNA to the solid support.

xi) More stringent washing condition is selected when

a) Probe has lower affinity with the DNA

b) Probe has higher affinity with the DNA

c) Probe is radio-labelled

d) Prove is non-radio-labelled/enzyme labelled.

xii) Microsatellites are

a) frequently found in bacterial genomes

b) always smaller than 50 bp

c) used as DNA marker

d) movable DNA elements.


( Short Answer Type Questions )

Answer any three of the following. [Marks 3x5 = 15]

2. Write a short note on c DNA library.

3. Write the basic differences between capillary transfer and

electro-transfer in sourthern blot.

4. Define cloning vector. What should be the properties of a

good vector ? [Marks 2 + 3]

5. Why Type-II restriction enzymes are considered to be the

most useful in molecular biology ? How it is different from

Type-I ? [Marks 3 + 2]

6. What is MCS in a vector ? Why alkaline phosphatase

treatment is needed in cloning ? What is linker ? How it

differs from adapters ? [Marks 1 + 2 + 1 + 1]

7. What is Directional Cloning ? When you use this ? Where

it is different from conventional cloning ? [Marks 2 + 1 + 2]

8. What do you mean by Restriction Modification System in

bacteria ? Explain.


( Long Answer Type Questions )

Answer any three of the following. [Marks 3x15 = 45]

9. What is the basic structure of plasmid vector ? Explain the

regulation of expression vector. What are steps of gene

cloning ? What are the characteristics of YAC and BAC ?

[Marks 3 + 5 + 3 + 4]

10. Write short notes on any three of the following : [Marks 3x5]

a) Sanger and Coulson method for DNA sequencing

b) Protein Blotting

c) Microarray

d) Inverse PCR.

11. What is Agarose ? PCR amplification is based on which

theory ? Briefly discuss the steps of PCR with suitable

diagram. Write down the applications of PCR. Briefly explain

the efficiency of PCR. [Marks 2 + 1 + 4 + 4 + 4]

12. What is a probe ? How they are classified ? What is Dot Blot

technique ? Briefly describe the technique of RFLP and

Asymmetric PCR. [Marks 2 + 3 + 4 + 6]

13. Write short notes on any three of the following : [Marks 3x5]

a) RAPD b) M13 vector

c) Blue-White screening

d) T-DNA transfer technique

e) Southern Blotting.


Maulana Abul Kalam Azad University of Technology, West Bengal
BF 142, Sector 1, Salt Lake City, Kolkata, West Bengal 700064
Answered By StudyChaCha Member
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