Go Back   2018-2019 StudyChaCha > StudyChaCha Discussion Forum > General Topics

Old February 1st, 2018, 10:20 AM
Posts: n/a
Default Wbutech Question Papers

I want the question paper of Molecular Genetics of B.Sc Microbiology, Genetics of Maulana Abul Kalam Azad University of Technology so can you provide me?
Reply With Quote Quick reply to this message
Other Discussions related to this topic
WBUTECH Registration
B.A.R.C sample papers and previous year question papers
IMU CET Question Papers
KSOU Question Papers MA English Question Papers
Tata Consultancy Services placement papers and aptitude question papers
LIC AAO question papers
MDU Question papers
VTU Question Papers 3rd SEM M.Tech Question Papers
CET Question Papers For MBA
Question papers of departmental test mainly panchayat test papers?
CBSE sample papers, model papers, question papers for class 12
PTU Question Papers of MBA 1st SEM
Aptitude question papers and Placement papers of TCS
VTU question papers 7th SEM ECE
WBUTech West Bengal University of Technology
CBSE question papers class XII question papers economics
UGC NET Question Papers
AAI Question Papers

Old February 1st, 2018, 10:21 AM
Educhamp's Avatar
Super Moderator
Join Date: May 2011
Location: Delhi
Posts: 38,230
Default Re: Wbutech Question Papers

I am providing you the question paper of Molecular Genetics of B.Sc Microbiology, Genetics of Maulana Abul Kalam Azad University of Technology

WBUT B.Sc Microbiology, Genetics Molecular Genetics question paper

Time Allotted
: 3 Hours Full Marks : 70

The figures in the margin indicate full marks.

Candidates are required to give their answers in their own words

as far as practicable.


( Multiple Choice Type Questions )

1. Choose the correct alternatives for any ten of the following :

[Marks 10x1 = 10]

i) Which of the following methods is most useful for

enzymatic amplification of specific segment of DNA ?

a) Nucleotide sequencing

b) DNA hybridization

c) PCR

d) None of these.

ii) Gene expression is analyzed by

a) Southern blot b) Restriction digestion

c) Northern blot d) None of these.

iii) In sourthern blotting experiment, the binding of

transferred DNA to the nylon membrane is ……………


a) ionic b) covalent

c) hydrophobic d) van der Waals.

iv) DNA hybridization is a technique, which relies on the

following properties of DNA, except

a) Double strandedness

b) sequence specificity

c) major and minor grooves

d) denaturation-renaturation properties.

v) For RNA detection which PCR is best ?

a) Nested PCR b) RT-PCR


vi) In Western blot the protein samples are run on

a) Agarose gel

b) polyacrylamide gel

c) formaldehyde-agarose gel

d) none of these.

vii) The stringency of a hybridization reaction depends on

all of the following, except

a) NaCl concentration

b) type of reporter molecule

c) nucleotide sequence of probe

d) pH.

viii) Ligase joins two DNA molecules together by forming a

covalent bond between

a) two PO4

b) two OH

c) one PO4 and one OH

d) Complementary nucleotides on opposite strands.

ix) -lactum ring is present in

a) Tetracycline b) Ampicillin

c) Kanamycin d) Streptomycin.

x) Principal function of reporter molecule in DNA

hybridization assay

a) enhance the stringency of hybridization reaction

b) aid in base pairing

c) aid in the detection of probe target hybridization

d) bind the target DNA to the solid support.

xi) More stringent washing condition is selected when

a) Probe has lower affinity with the DNA

b) Probe has higher affinity with the DNA

c) Probe is radio-labelled

d) Prove is non-radio-labelled/enzyme labelled.

xii) Microsatellites are

a) frequently found in bacterial genomes

b) always smaller than 50 bp

c) used as DNA marker

d) movable DNA elements.


( Short Answer Type Questions )

Answer any three of the following. [Marks 3x5 = 15]

2. Write a short note on c DNA library.

3. Write the basic differences between capillary transfer and

electro-transfer in sourthern blot.

4. Define cloning vector. What should be the properties of a

good vector ? [Marks 2 + 3]

5. Why Type-II restriction enzymes are considered to be the

most useful in molecular biology ? How it is different from

Type-I ? [Marks 3 + 2]

6. What is MCS in a vector ? Why alkaline phosphatase

treatment is needed in cloning ? What is linker ? How it

differs from adapters ? [Marks 1 + 2 + 1 + 1]

7. What is Directional Cloning ? When you use this ? Where

it is different from conventional cloning ? [Marks 2 + 1 + 2]

8. What do you mean by Restriction Modification System in

bacteria ? Explain.


( Long Answer Type Questions )

Answer any three of the following. [Marks 3x15 = 45]

9. What is the basic structure of plasmid vector ? Explain the

regulation of expression vector. What are steps of gene

cloning ? What are the characteristics of YAC and BAC ?

[Marks 3 + 5 + 3 + 4]

10. Write short notes on any three of the following : [Marks 3x5]

a) Sanger and Coulson method for DNA sequencing

b) Protein Blotting

c) Microarray

d) Inverse PCR.

11. What is Agarose ? PCR amplification is based on which

theory ? Briefly discuss the steps of PCR with suitable

diagram. Write down the applications of PCR. Briefly explain

the efficiency of PCR. [Marks 2 + 1 + 4 + 4 + 4]

12. What is a probe ? How they are classified ? What is Dot Blot

technique ? Briefly describe the technique of RFLP and

Asymmetric PCR. [Marks 2 + 3 + 4 + 6]

13. Write short notes on any three of the following : [Marks 3x5]

a) RAPD b) M13 vector

c) Blue-White screening

d) T-DNA transfer technique

e) Southern Blotting.


Maulana Abul Kalam Azad University of Technology, West Bengal
BF 142, Sector 1, Salt Lake City, Kolkata, West Bengal 700064
Answered By StudyChaCha Member
Reply With Quote Quick reply to this message
Our Latest Videos
Sponsored Links


Reply to this Question / Ask Another Question
Your Username: Click here to log in


Forum Jump

All times are GMT +6.5. The time now is 08:07 PM.

Powered by vBulletin® Version 3.8.11
Copyright ©2000 - 2018, vBulletin Solutions Inc.
Search Engine Friendly URLs by vBSEO 3.6.0 PL2

1 2 3 4 5 6 7 8 9