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Will you please provide me you question paper for the GATE entrance examination

Here I am giving you question paper for the GATE entrance examination in PDF file attached with it so you can get it free of cost ..

Some content of PDF file is given below ..

GATE biotechnology paper “::
Q. 1 – Q. 25 carry one mark each.
Q.1 In mismatch correction repair, the parental DNA strand is distinguished from the daughter strand by
(A) acetylation (B) phosphorylation (C) methylation (D) glycosylation

Q.2 The basis for blue-white screening with pUC vectors is
(A) intraallelic complementation (B) intergenic complementation
(C) intragenic suppression (D) extragenic suppression

Q.3 Idiotypic determinants of an antibody are associated with the
(A) constant region of the heavy chains (B) constant region of the light chains
(C) variable region (D) constant regions of light and heavy chains

Q.4 Identification of blood groups involves
(A) precipitation (B) neutralization
(C) opsonization (D) agglutination

Q.5 B-lymphocytes originate from the bone marrow whereas T-lymphocytes originate from
(A) thymus (B) bone marrow (C) spleen (D) liver

Q.6 A humanized antibody is one in which the
(A) heavy and light chains are from human
(B) heavy chain is from human and light chain is from mouse
(C) light chain is from human and heavy chain is from mouse
(D) CDRs are from mouse, and the rest is from human

Q.7 Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because
(A) it is an organic solvent
(B) it easily penetrates cells
(C) it protects cells by preventing crystallization of water
(D) it is also utilized as a nutrient

Q.8 Nude mice refers to
(A) mice without skin (B) mice without thymus
(C) knockout mice (D) transgenic mice

Q.9 Heat inactivation of serum is done to inactivate
(A) prions (B) mycoplasma (C) complement (D) pathogenic bacteria

Q.10 Choose the correct signal transduction pathway.
(A) Hormone → 7 TM receptor → G protein → cAMP → PKA
(B) Hormone → G protein → 7 TM receptor → cAMP → PKA
(C) Hormone → 7 TM receptor → G protein → PKA → cAMP
(D) Hormone → 7 TM receptor → cAMP → G protein → PKA

Q.11 A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be
generated by substituting that serine with
(A) glycine (B) alanine (C) aspartate (D) threonine

Q.12 A truncated polypeptide is synthesized due to a nonsense mutation. Where would you introduce
another mutation to obtain a full-length polypeptide?
(A) Ribosomal protein gene (B) Transfer RNA gene
(C) DNA repair gene (D) Ribosomal RNA gene

Q.13 Protein-DNA interactions in vivo can be studied by
(A) gel shift assay (B) Southern hybridization
(C) chromatin immunoprecipitation assay (D) fluorescence in situ hybridization assay

Attached Files Available for Download

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