Molecular micro biology syllabus of HCU

hello,
I want to know syllabus for molecular microbiology entrance (in HCU )

University of Hyderabad Molecular Microbiology subject is a part of M.Sc Biochemistry, here are detailed syllabuses:

Molecular icrobiology

1. Discovery of DNA. Early experiments in molecular genetics. Historical events that lead to the conclusion of DNA is the genetic material. [3 hours]

2. Structure of DNA and RNA. Chemical and physical properties of nucleic acids (stability of nucleic acids, buoyant density, purity of DNA, effect of acids, alkali, on DNA, viscosity, spectroscopic and thermal properties of nucleic acids). [3 hours]

3. Genome Analysis and complexity, Cot analysis, organization of protein coding genes, gene duplication, discovery of repetitious DNA fractions. Lines, Sines and Alu sequences. [2 hours]

4. Chromosomes, Chromatin and the nucleosome. Chromosome sequence, genome size, density and diversity; duplication and segregation; building blocks of chromosomes or nucleosomes, higher order structure and regulation of chromatin structure. [2 hours]

5. DNA replication in prokaryotes and eukaryotes: origin of replication, replication fork, replisome. Enzymes in DNA synthesis, structure, function and mechanisms of action. Methods for studying DNA replication and determination of origin of replication. Chromosome segregation: random versus biased segregation. Topological problems during replication. DNA supercoiling and topoisomerases in eukaryotes and prokaryotes. Mechanisms of actions of topoisomerases. [6 hours]

6. Mutations: Replication errors in DNA, chemical mutagens, spontaneous versus induced mutation. Types of DNA damages. Transposons and mechanisms of transposition. [3 hours]

7. DNA repair: direct repair system, excision repair (NER and BER), Mismatch repair (MMR), double stranded DNA break repair (DSB): non-homologous end joining and homologous recombination. [4 hours]

8. Biochemistry of Recombination; types of homologous recombination: Gene conversion and mating type switching, Site-specific recombination, VD-J recombination, applications of homologous recombination. [3 hours]

9. Recombinant DNA technology: Restriction digestion; applications of DNA polymerases and PCR.; DNA modifying enzymes in cloning; DNA sequencing; Cloning vectors and hosts, gene libraries, Screening libraries. [6 hours]








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