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What is the selection procedure of the ICAR NET prelims and Main Examination?
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Indian Council of Agricultural Research (ICAR) is the largest scientific organization of India. ICAR NET Exam determines the eligibility for Lecturer or Assistant professor. Preliminary Examination: The preliminary examination consists of 150 objective type questions. Minimum of 60% marks from General/ OBC category and 50% marks from SC/ ST/ PH category to qualify the exam. Candidates qualifying in the same will only be allowed to appear in the ARS – Main Examination. Marks scored in the prelims will not be counted for final selection. Main Examination: The Main paper will have paper of 240 questions consisting of three sections A, B, and C to be completed in 3 hours duration. Part A will consist of 40 questions carrying 2 marks each Part B will have 20 questions of 5 marks each Part C will have 6 essay type or descriptive type questions of 10 marks each. Minimum of 55% marks from General/ OBC category and 50% marks from SC/ ST/ PH category to qualify the exam. Candidates qualifying in the Main Exam will be called for Viva Voce. The candidates will be called in the ratio 1:5 subject to minimum of 55% obtained by General/ OBC category candidates and 50% marks by SC / ST / PC candidates in ARS Mains Examination (Subjective type). Final Selection: The final list will be prepared on the basis of marks obtained in the ARS – Mains Examination and Viva-voce and the candidates. The number of attempts for NET Examination is limited to % for each and every candidate.
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Agricultural Scientists Recruitment Board (ASRB) is conducts a National Eligibility Test (NET) to examine the eligibility for the post of Lecturer/ Assistant Professor in agricultural universities. ICAR NET exam There are only one paper will conduct of 150 marks. The question paper will consist total 150 multiple choice objective type questions. The duration of the exam will be two hours. There is a negative marking of 1/3 mark for every wrong answers. Required marks toqualifying ICAR NET examination 2015: Category Percentage of marks (UR) (General) /OBC(Creamy layer) 55% OBC(Non-Creamy layer) 50% SC / ST/ Physically Challenged(PC) Person 45% Question paper will be set in both languages Hindi and English. ICAR NET exam pattern Preliminary Exam: 150 Marks (Objective Type Questions) in 2 hrs duration (Negative Marking: 1/3rd) Mains Exam: 240 Marks (Descriptive Type Questions) in 3 hrs duration Viva Voce: 60 Marks Syllabus of NET 01. Agricultural Biotechnology Unit 1: Cell Structure and Function Prokaryotic and eukaryotic cell architecture, Cell wall, plasma membrane, Structure and function of cell organelles: vacuoles, mitochondria, plastids, golgi apparatus, ER, peroxisomes, glyoxisomes. Cell division, regulation of cell cycle, Protein secretion and targeting, Cell division, growth and differentiation. Unit 2: Biomolecules and Metabolism Structure and function of carbohydrates, lipids, proteins and nucleic acids, Synthesis of carbohydrate, glycolysis, HMP, Citric acid cycle and metabolic regulation,Oxidative phosphorylation and substrate level phosphorylation, Vitamins, plant and animal hormones. Functional molecules, antioxidants, nutrient precursor, HSPs, anti-viral compounds. Unit 3: Enzymology Enzymes, structure conformation, classification, assay, isolation, purification and characterization, catariysis specificity, mechanism of action, active site, regulation of enzyme activity, multienzyme complexes, immobilized enzymes and protein engineering, immobilized enzymes and their application. Unit 4: Molecular Genetics Concept of gene, Prokaryotes as genetic system, Prokaryotic and eukaryotic chromosomes, methods of gene isolation and identification, Split genes, overlapping genes and pseudo genes, Organization of prokaryotic and eukaryotic genes and genomes including operan, exon, intron, enhancer promoter sequences and other regulatory elements. Mutation – spontaneous, induced and site-directed, recombination in bacteria, fungi and viruses, transformation, transduction, conjugation, transposable elements and transposition. Unit 5: Gene Expression Expression of genetic information, operon concept, Transcription – mechanism of transcription in prokaryotes and eukaryotes, transcription unit, regulatory sequences and enhancers, activators, repressors, co-activators, Co-repressors in prokaryotes and eukaryotes, inducible genes and promoters, Transcription factors post transcriptional modification and protein transport, DNA-protein interaction, Genetic code. Mechanism of translation and its control, post translational modifications. Unit 6: Molecular Biology Techniques Isolation and purification of nucleic acids. Nucleic acids hybridization: Southern, northern and western blotting hybridization. Immune response monoclonal and polyclonal antibodies and ELISA, DNA sequencing. Construction and screening of genomic and C-DNA libraries. Gel electrophoretic techniques. Polymerase chain reactor spectroscopy, rtPCR ultracentrifugation, chromatography, FISH, RIA etc. Unit 7: Gene Cloning Restriction enzymes and their uses. Salient features and uses of most commonly used vectors i.e. plasmids, bacteriophages, phagmids, cosmids, BACs, PACs and YACs, binary vectors, expression vectors. Gene cloning and sub-cloning strategies, chromosome walking, genetic transformation, Basis of animal cloning. Biology. Risk assessment and IPR. Unit 8: Molecular Biology Ribosome structure and function. Protein biosynthesis in prokaryotes and ekaryotes. Post-translational modification. Gene regulation, RNA processing and Post transcriptional modifications. Bioprospecting, biofortification, gene pryrimiding and gene fusion, nbozyme technology. Unit 9: Plant Molecular Biology Photoregulation and phytochrome regulation of nuclear and chloroplastic gene expression. Molecular mechanism of nitrogen fixation. Molecular biology of various stresses, viz. abiotic stresses like drought, salt, heavy metals and temperature; and biotic stresses like bacterial, fungal and viral diseases. Signal transduction and its molecular basis, molecular mechanism of plant hormone action mitochondrial control of fertility, structure, organization and regulation of nuclear gene concerning storage proteins and starch synthesis. Unit 10: Tissue Culture Basic techniques in cell culture and somatic cell genetics. Regulation of cell cycle and cell division. Clonal propagation. Concept of cellular totipotency. Anther culture, somaclonal and gametoclonal variations. Hybrid embryo culture and embryo rescue, somatic hybridization and cybridization. Application of tissue culture in crop improvement. Secondary metabolite production. In vitro, mutagenesis, cryopreservation and plant tissue culture repository. Unit 11: Plant Genetic Engineering Isolation of genes of economic importance. Gene constructs for tissue-specific expression. Different methods of gene transfer to plants, Potential applications of plant genetic engineering for crop improvement, i.e. insect-pest resistance (insect, viral, fungal and bacterial disease resistance), abiotic stress resistance, herbicide resistance, storage protein quality, increasing shelf-life, oil quality, Current status of transgenics, biosafty norms and controlled field trials and release of transgenics (GMOs). Unit 12: Molecular Markers and Genomics DNA molecular markers: Principles, type and applications; restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), randomly amplified polymorphic DNA sequences (RAPD), Simple sequence repeats (SSR), Single nucleotide polymorphism (SNP), Structural and functional genomics, gene mapping, genome mapping, gene tagging and comparative genomics and application of genomics.
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