Thiruvalluvar University MSC Biotechnology Syllabus

I have taken admission at Thiruvalluvar University for MSC Biotechnology Program. So is there any one who will provide syllabus of MSC Biotechnology Program?

As you need syllabus of MSC Biotechnology Program of Thiruvalluvar University, so here is following syllabus:

Thiruvalluvar University MSC Biotechnology Syllabus

SEM I
Advanced Biochemistry
Cell and Molecular Biology
Microbiology and Microbial Technology
Core Practical (Out of Papers I, II and III)
Natural Products and Intellectual Property Rights

SEM II
Biophysics, Bio-statistics and Computer Applications
Plant Molecular Biotechnology
Animal Cell Culture Technology
Core Practical (Out of Papers I, II and III)
Core Practical (Out of Papers V and VI)
Human Rights
Bioinformatics

SEM III
Recombinant DNA Technology
Immunology and Immunotechnology
Enzyme and Enzyme Technology
Core Practical (Out of Papers VII, VIII and IX)
Bio Processing Technology
Vermi-CompostingTechnology

SEM IV
Research Methodology
Core Practical (Out of Papers VII, VIII and IX)
Core Practical course: Research Methodology
Biotechnology, Bio-safety and Bio-ethics
Core Project/Dissertation with viva voce

Thiruvalluvar University MSC Biotechnology Syllabus
I SEMESTER
PAPER I ADVANCED BIOCHEMISTRY
UNIT-I
Chemical foundations of biology - pH, pK, acids, bases, ionic bonds, covalent bonds and secondary bonds (hydrogen bonds and Vander Waal’s bonds). Classes of organic compounds and functional groups - atomic and molecular dimensions, space filling and ball and stick models.
UNIT-II
Definition, nomenclature, classification, structure, chemistry and properties of Carbohydrates, Proteins, Amino acids, Lipids and Nucleic acids - Polynucleotides, protein like haemoglobin, myoglobin and plasma proteins - classification of porphyrines: structure and properties - structure of metalloporphyrines- haeme and chlorophyll.
UNIT-III
Metabolism of Carbohydrates, Proteins, Amino acids, Lipids and Nucleic acids-their biosynthesis and degradation; mechanism of oxidative phosphorylation and its inhibitors, and photophosphorylation, urea cycle, hormonal regulation of mammalian metabolism.
UNIT-IV
Heterocylic compounds and secondary metabolites - prostaglandins, leukotrienes, thromboxanes, interferons and interleukins, antibodies, alkaloids, plant and animal pigments.
Isolation and separation techniques: chromatography - gas chromatography and high - performance liquid chromatography.
UNIT-V
Enzymes: general aspects (classification and structure), allosteric mechanism, regulatory and active sites, isoenzymes, enzyme kinetics, enzymatic catalysis.
Cell fractionation by Agarose and Polyacyrlamide gel electrophoresis, disc and slab - gel electrophoresis and by differential centrifugation - ultra-centrifugation, density gradient centrifugation.
Books for Reference
1. Biochemistry, 4th edition, L.Stryer., 1999. W.H, Freeman & company, New York.
2. Molecular Biomethods Handbook, R.Rapley & J.M. Walker, 1998. Humana press.
3. Principles of Biochemistry, AL. Lehninger, D.L. Nelson and M. M. Cox., 1993. Worth Publishers, New York.
4. Biochemistry 4th edition, G. Zubay, 1998. Mc Millan Publishing Co.

New York.
LAB IN ADVANCED BIOCHEMISTRY
1. pH measurements and preparation of buffers.
2. Determination of Chl.a, Chl.b & total Chl. by Arnon method.
3. Qualitative tests for carbohydrates, alkaloids, terpenoids, tannins, fatty acids, phenolics, and volatile oils.
4. Estimation of total soluble sugars.
5. Estimation of proteins by Lowry’s method.

6. Determination of saponification number of lipids.
7. Estimation of amino acids.
8. Separation and identification of sugars and amino acids by chromatography.
9. Biochemical estimation of DNA/RNA using spectrophotometer.
10. Determination of amylase, peroxidase, catalase activity using spectrophotometer.

Reference
1. Physiology Chemistry, Oser.B.L.Hawks, 1965. TATA McGrew Hill.
2. Laboratory manual in biochemistry, Strolve, B.L.A., Mzka vora, V.C., 1989. MIR Publisher, Moscow.
3. Biochemical methods, Sadasivam and Manikam, 1996. New age international publishers, Second Edition. New Delhi
4. Biochemical methods, Sridhar and Mahadevan. Madras University, Chennai.

PAPER II CELL AND MOLECULAR BIOLOGY
UNIT-I
The dynamic cell - the molecules of life - the architecture of cells - cell cycle events - cells into tissues - molecular cell biology - Protein structure and function - Hierarchical structure of proteins - folding, modification and degradation of proteins - functional design of proteins - membrane proteins - purification, detection, and characterization of proteins.
UNIT-II
Molecular structures, biosynthesis of macromolecules (proteins, polysaccharides, nucleic acids) - Biomembranes and subcellular organization of eukaryotic cells - microscopy and cell architecture - purification of cells and their parts - biomembranes organelles of eukaryotic cell.
UNIT-III
Nucleus (structure including ultra structure and functions) - chromosome structure and types - Programmed Cell Death (PCD), DNA - Denaturation and renaturation, C-value paradox and the reasons, circular and spherical DNA - Gene amplification - PCR, DNA finger printing - DNA replication - polymerases, primers and mechanism - molecular methods of DNA replication.
UNIT-IV
RNA - types - molecular organization - genetic code - transcription mechanism in prokaryotes and post transcription processing - enzyme system in transcription - transcription process in eukaryotes.
UNIT-V
Cell signaling - communication between the cells and their environment - characteristics of the cell signaling system, Second messenger (plant and animal cells)
and G-protein coupled receptors, receptors of tyrosine kinases - signals that originate from contact between cell structure and substratum, convergence, divergence and cross talk among different signaling pathways.
Books for Reference
1. Cell and molecular biology-Concept and experiment. 2nd edn., Harris,D(Ed.), Karp, G.1999. John wiley & sons, sons, New York.
2. Principles of cell and molecular biology. 2nd edn., Mclaughlin,S., Trost,K., Mac Elree,E.(eds)., Kleinsmith,L.J.& Kish, V.M., 1995. Harper Collins Publisher, New York.
3. Molecular biology of the cell.3nd edn., Alberts,B., Bray,D., Lawis,J., Raff,M., Roberta, K., Watson, J.d(eds.), 1994. Garland Publication, Inc., New York.
4. Cell and Molecular Biology. 8th edn., De Robertis, E.D.P. and De Roberts, E, M.F.1995. B.I.Waverly Pvt. Ltd., New Delhi.
5. Introduction to Molecular Biology, Peter Paolella, 1998. Mc Graw-Hill.
6. Molecular Cell Biology, Media connected W.H.Freeman and company.

LAB IN CELL AND MOLECULAR BIOLOGY
1. Observation of prokaryotic and eukaryotic cells and cell types - Living Cells/Temporary/Permanent Preparations.
2. Study of cell organelles adopting preparations/models.
3. Squash preparation of giant chromosome of salivary gland of chironomous larva.
4. Squash preparation of onion root tip, testis and anther lobes.
5. Preparation of buccal smear.
6. Red blood cell as osmometer.
7. Subcellular fractionation and biochemical/enzymological analysis.
8. Cytochemical study of cells/cell types using specific dyes/reagents.
9. Immunocytochemical analysis for specific cellular constituents.
10. Metaphase chromosome preparations and preliminary banding techniques.
11. Isolation, determination, purification and separation of protein, carbohydrates, lipids, DNA and RNA.

PAPER III
MICROBIOLOGY AND MICROBIAL TECHNOLOGY
UNIT-I
Classification of microorganism - Kingdom - protista, prokaryotic and eukaryotic microorganisms, the five-kingdom concept of classification, archaeobacteria, eubacteria and eukaryotes; History of Microbiology;
Microscopy - light, electron and laser optic system; micrometry.
UNIT-II
Prokaryotic and Eukaryotic cell structures; pure culture techniques- isolation, cultivation, enumeration and preservation of microbes; staining techniques- simple and differential staining.
Nutritional requirements and nutritional grouping of microorganisms; Different media (simple, complex and defined) - Growth curve; Axenic culture, Synchronous culture, Continuous culture; Different; Effects of physical and chemical factors on microbial growth.
Microbial genetics-recombination - transformation, transduction, conjugation, regulation of gene expression.
UNIT-III
Microbial diversity - Methods to assess microbial diversity, Merits and demerits of culture dependent and culture independent methods. Molecular analysis of bacterial community: Denaturing Gradient Gel Electrophoresis (DGGE), Terminal Restriction Fragment Length Polymorphism (T-RFLP), Amplified Ribosomal DNA and Restriction Analysis (ARDRA).
UNIT-IV
Microbes in natural habitats - air, water & soil. Industrial application of microbes - Wine, Beer, Cheese, Yogurt. Primary and secondary metabolites and their applications; preservation of food; biogas; bio-fertilizers and bio-pesticides; leaching of ores by microorganisms; microorganisms and pollution control-bioremediation; biosensors.
UNIT-V
Microbial Pathogenicity - toxins, mode of action. Bacterial pathogens - Staphylococcus, Streptococcus, Escherichia, Salmonella & Mycobacterium. Viral pathogens - Influenza, Rabies, Enterovirus, Retrovirus, Oncogenic viruses.
Control of microorganisms - physical and chemical methods - antibiotics and chemotherapeutic agents - anti microbial susceptibility test.
Books for Reference
1. Microbiology, L.M. Prescott, J.P. Harley and D.A. Klein, 7/e, 2007. McGraw Hill, Boston.
2. Microbiology, L.M. Prescott, J.P. Harley and D.A. Klein, 6/e, 2005. McGraw Hill, Boston.
3. Fundamental Principles of Bacteriology, A.J. Salle, 1999. Tata McGraw - Hill Publishing Company Limited, New Delhi.
4. Medical Microbiology, D. Greenwood, R. Slack and J. Peutherer, 1997. ELST with Churchill Livingstone, Hong Kong.
5. Microbial Ecology. Fundamentals and Applications, R. M. Atlas and R. Bartha, 2000.
6. Microbiology, M.J. Pelzer Jr., E.C.S. Chan and N.R. Kreig, 1993. McGraw Hill Inc., New York.
7. Microbial Functional Genomics, J.Zhou, D.K. Thomson. Y.Xu. J.M. Tiedje. J.Wiley, 2004.

LAB IN MICROBIOLOGY AND MICROBIAL TECHNOLOGY
1. Sterilization and preparation of media, Enumeration of bacteria and fungi from environmental samples - soil, water and air. Techniques for pure culture - streaking, pour plate and spread plate.
2. Stains and staining techniques, simple staining, negative staining & differential staining techniques. Motility studies.
3. Bacterial growth - Growth curve, factors affecting bacterial growth - pH, Temperature and Salinity.
4. Biochemical tests for identification of bacteria.
5. Antimicrobial assay, phenol coefficient, agar plate sensitive method.

6. Cultivation and morphology of molds and yeast
7. Microbiological analysis of urine and blood specimens
8. Water quality analysis - MPN method.
9. Microbial analysis of food samples, methylene blue reduction test for milk.
10. Microbial production of food and beverages by fermentation-wine and yogurt
11. Isolation of UV-mutant, isolation of antibiotic resistant strains and tryptophan mutant. Ames test to screen for chemical carcinogens.

Books for Reference
1. Microbiology: A Laboratory Manual, J.G. Cappuccino and N. Sherman, 2002. Addison-Wesley.
2. Laboratory Manual of Experimental Microbiology, R.M. Atlas, A.E.
Brown and L.C. Parks, 1995. Mosby, St. Louis.
3. Laboratory Manual in General Microbiology, N. Kannan, 2002. Panima
Publishers.
4. Bergey’s Manual of Determinative Bacteriology. Ninth edition J.G.Holt, N.R.Krieg, Lippincott Williams, 2000. Wilkin Publishers.

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