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January 26th, 2014 06:45 PM
Re: Last year GATE Biotechnology Question Papers

As you require the last year GATE Biotechnology question paper, so here I am sharing the same with you

Q.1 Under alkaline conditions, DNA is more stable than RNA because
(A) RNA forms secondary structures
(B) RNA is a single stranded molecule
(C)RNA has uracil in place of thymidine
(D)RNA is susceptible to hydrolysis

Q.2 Which one of the following modifications is common toboth protein and DNA?
(A) SUMOylation (B) Nitrosylation (C)Methylation (D) Ubiquitination

Q.3 Protein A, which has strong affinity to Fc region of immunoglobulin, is extracted from
(A)Saccharomyces cerevisiae
(B)Staphylococcus aureus
(C)Streptococcus pyogenes
(D)Streptococcus sanguis

Q.4 The first humanized monoclonal antibody approved for the treatment of breast canceris
(A) Rituximab (B) Cetuximab (C) Bevacizumab (D) Herceptin

Q.5 Which one of the following aminoacids in proteins does NOT undergo phosphorylation?
(A) Ser (B) Thr (C) Pro (D) Tyr

Q.6 The role of an adjuvant isto
(A) prolong the persistence of antigen
(B) cross link the antigen
(C) increase the size of antigen
(D) avoid inflammation

Q.7 Endogenous antigens are presented on to the cell surface along with
(A) MHC-II (B) MHC-I (C) Fcγreceptor (D) complement receptor

Q.8 Human genome sequencing project involved the construction of genomic library in
(A)bacterial artificial chromosome (B)pBR322
(C) bacteriophage (D) pcDNA3.1

Q.9 The nucleotide analogue used in DNA sequencing by chain termination method is
(A) 1',3'-dideoxy nucleoside triphosphate
(B) 2',3'-dideoxy nucleoside triphosphate
(C) 2',4'-dideoxy nucleoside triphosphate
(D) 2',5'-dideoxy nucleoside triphosphate

Q.10 Choose the correct signal transduction pathway.
(A) Hormone → 7 TM receptor → G protein → cAMP → PKA
(B) Hormone → G protein → 7 TM receptor → cAMP → PKA
(C) Hormone → 7 TM receptor → G protein → PKA → cAMP
(D) Hormone → 7 TM receptor → cAMP → G protein → PKA

Q.11 A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be
generated by substituting that serine with
(A) glycine (B) alanine (C) aspartate (D) threonine

Q.12 A truncated polypeptide is synthesized due to a nonsense mutation. Where would you introduce
another mutation to obtain a full-length polypeptide?
(A) Ribosomal protein gene (B) Transfer RNA gene
(C) DNA repair gene (D) Ribosomal RNA gene

Q.13 Protein-DNA interactions in vivo can be studied by
(A) gel shift assay (B) Southern hybridization
(C) chromatin immunoprecipitation assay (D) fluorescence in situ hybridization assay

Q.14 The direction of shell coiling in the snail Limnaea peregra is a classic example of
(A) chromosomal inheritance (B) extra-chromosomal inheritance
(C) chromosomal translocation (D) homologous recombination

Q.15 During photorespiration under low CO2 and high O2 levels, O2 reacts with ribulose
1,5- bisphosphate to yield
(A) one molecule each of 3-phosphoglycerate and 2-phosphoglycolate
(B) two molecules of 3-phosphoglycerate
(C) two molecules of 2-phosphoglycolate
(D) one molecule each of 3-phosphoglycerate and glyoxylate

Q.16 Which one of the following is NOT a protoplast fusion inducing agent?
(A) Inactivated Sendai virus (B) Ca2+ at alkaline pH
(C) Polyethylene glycol (D) Colchicine

Q.17 The activity of an enzyme is expressed in International Units (IU). However, the S.I. unit for
enzyme activity is Katal. One Katal is
(A) 1.66 × 104 IU (B) 60 IU (C) 6 × 107 IU (D) 106 IU

Q.18 Identify the statement that is NOT applicable to an enzyme catalyzed reaction.
(A) Enzyme catalysis involves propinquity effects
(B) The binding of substrate to the active site causes a strain in the substrate
(C) Enzymes do not accelerate the rate of reverse reaction
(D) Enzyme catalysis involves acid-base chemistry

Q.19 An example of a derived protein structure database is
(A) Pfam (B) SCOP (C) GEO (D) Prosite

Q.20 An example of a program for constructing a phylogenetic tree is
(A) phylip (B) phrap (C) prodom (D) PHDsec

Q.21 Synteny refers to
(A) gene duplication from a common ancestor
(B) a tree representation of related sequences
(C) the extent of similarity between two sequences
(D) local conservation of gene order

Q.22 While searching a database for similar sequences, E value does NOT depend on the
(A) sequence length (B) number of sequences in the database
(C) scoring system (D) probability from a normal distribution

Q.23 In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen
(A) ferrous ammonium sulfate (B) uranium acetate
(C) sodium chloride (D) basic fuchsin

Q.24 The molarity of water in a water : ethanol mixture (15 : 85, v/v) is approximately
(A) 0.85 (B) 5.55
(C) 8.5 (D) 55.5

Q.25 The helix content of a protein can be determined using
(A) an infrared spectrometer (B) a fluorescence spectrometer
(C) a circular dichroism spectrometer (D) a UV-Visible spectrophotometer

Q. 26 to Q. 55 carry two marks each.
Q.26 Which one of the following DNA sequences carries an invert repeat?

Q.27 In zinc finger proteins, the amino acid residues that coordinate zinc are
(A) Cys and His (B) Asp and Glu
(C) Arg and Lys (D) Asp and Arg

Q.28 Match the entries in Group I with those in Group II.
Group I Group II
P. MTT 1. Dihydrofolate reductase
Q. Annexin V 2. Succinate dehydrogenase
R. Methotrexate 3. Microtubules
S. Taxol 4. Phosphatidylserine
(A) P-3, Q-1, R-4, S-2 (B) P-2, Q-4, R-1, S-3
(C) P-2, Q-3, R-4, S-1 (D) P-4, Q-2, R-1, S-3

Q.29 In an exponentially growing batch culture of Saccharomyces cerevisiae, the cell density is 20 gl-1
(DCW), the specific growth rate () is 0.4 h-1 and substrate uptake rate () is 16 gl-1h-1. The cell
yield coefficient Yx/s will be
(A) 0.32 (B) 0.64 (C) 0.80 (D) 0.50

Q.30 A single base pair of DNA weighs 1.1 × 10-21 grams. How many picomoles of a plasmid vector of
length 2750 bp are contained in 1 g of purified DNA?
(A) 0.30 (B) 0.55 (C) 0.25 (D) 0.91

Rest of the Questions are attached in below file which is free of cost
January 26th, 2014 05:50 PM
AdityaV Here I am searching for the last year GATE Biotechnology question papers, can you please help me in my searching???

Here I am giving you Syllabus and Last Year’s Question Papers of GATE (Graduate Aptitude Test in Engineering) Biotechnology.

Syllabus for GATE Biotechnology:

Engineering Mathematics:

Linear Algebra: Matrices and determinants, Systems of linear equations, Eigen values and Eigen vectors.

Calculus: Limit, continuity and differentiability, Partial derivatives, Maxima and minima, Sequences and series, Test for convergence, Fourier Series.

Differential Equations: Linear and nonlinear first order ODEs, higher order ODEs with constant coefficients, Cauchy’s and Euler’s equations,Laplace transforms, PDE- Laplace, heat and wave equations.

Probability and Statistics: Mean, median, mode and standard deviation, Random variables, Poisson, normal and binomial distributions, Correlation and regression analysis.

Numerical Methods: Solution of linear and nonlinear algebraic equations, Integration of trapezoidal and Simpson’s rule, Single and multistep methods for differential equations.


Microbiology: Prokaryotic and eukaryotic cell structure; Microbial nutrition, growth and control; Microbial metabolism (aerobic and anaerobic respiration, photosynthesis); Nitrogen fixation; Chemical basis of mutations and mutagens; Microbial genetics (plasmids, transformation, transduction, conjugation); Microbial diversity and characteristic features; Viruses.

Biochemistry: Biomolecules and their conformation; Weak inter-molecular interactions in biomacromolecules; Chemical and functional nature of enzymes; Kinetics of single substrate and bi-substrate enzyme catalyzed reactions; Bioenergetics; Metabolism (Glycolysis, TCA and Oxidative phosphorylation); Membrane transport and pumps; Cell cycle and cell growth control; Cell signaling and signal transduction.

Molecular Biology and Genetics: Molecular structure of genes and chromosomes; DNA replication and control; Transcription and its control; Translational processes; Regulatory controls in prokaryotes and eukaryotes; Mendelian inheritance; Gene interaction; Complementation; Linkage, recombination and chromosome mapping; Extrachromosomal inheritance; Chromosomal variation; Population genetics; Transposable elements, Molecular basis of genetic diseases and applications.

Process Biotechnology: Bioprocess technology for the production of cell biomass and primary/secondary metabolites, such as baker’s yeast, ethanol, citric acid, amino acids, exo-polysacharides, antibiotics and pigments etc.; Microbial production, purification and bioprocess application(s) of industrial enzymes; Production and purification of recombinant proteins on a large scale; Chromatographic and membrane based bioseparation methods; Immobilization of enzymes and cells and their application for bioconversion processes.

Aerobic and anaerobic biological processes for stabilization of solid / liquid wastes; Bioremediation.

Bioprocess Engineering: Kinetics of microbial growth, substrate utilization and product formation; Simple structured models; Sterilization of air and media; Batch, fed-batch and continuous processes; Aeration and agitation; Mass transfer in bioreactors; Rheology of fermentation fluids; Scale-up concepts; Design of fermentation media; Various types of microbial and enzyme reactors; Instrumentation in bioreactors.

Plant and Animal Biotechnology: Special features and organization of plant cells; Totipotency; Regeneration of plants; Plant products of industrial importance; Biochemistry of major metabolic pathways and products; Autotrophic and heterotrophic growth; Plant growth regulators and elicitors; Cell suspension culture development: methodology, kinetics of growth and production formation, nutrient optimization; Production of secondary metabolites by plant suspension cultures; Hairy root cultures and their cultivation. Techniques in raising transgencies.

Characteristics of animal cells: Metabolism, regulation and nutritional requirements for mass cultivation of animal cell cultures; Kinetics of cell growth and product formation and effect of shear force; Product and substrate transport; Micro & macro-carrier culture; Hybridoma technology; Live stock improvement; Cloning in animals; Genetic engineering in animal cell culture; Animal cell preservation.

Immunology: The origin of immunology; Inherent immunity; Humoral and cell mediated immunity; Primary and secondary lymphoid organ; Antigen; B and T cells and Macrophages; Major histocompatibility complex (MHC); Antigen processing and presentation; Synthesis of antibody and secretion; Molecular basis of antibody diversity; Polyclonal and monoclonal antibody; Complement; Antigen-antibody reaction; Regulation of immune response; Immune tolerance; Hyper sensitivity; Autoimmunity; Graft versus host reaction.

Recombinant DNA Technology: Restriction and modification enzymes; Vectors: plasmid, bacteriophage and other viral vectors, cosmids, Ti plasmid, yeast artificial chromosome; cDNA and genomic DNA library; Gene isolation; Gene cloning; Expression of cloned gene; Transposons and gene targeting; DNA labeling; DNA sequencing; Polymerase chain reactions; DNA fingerprinting; Southern and northern blotting; In-situ hybridization; RAPD; RFLP; Site-directed mutagenesis; Gene transfer technologies; Gene therapy.

Bioinformatics: Major bioinformatics resources (NCBI, EBI, ExPASy); Sequence and structure databases; Sequence analysis (biomolecular sequence file formats, scoring matrices, sequence alignment, phylogeny); Genomics and Proteomics (Large scale genome sequencing strategies; Comparative genomics; Understanding DNA microarrays and protein arrays); Molecular modeling and simulations (basic concepts including concept of force fields).

Last Year’s Question Papers of GATE Biotechnology:

GATE Biotechnology Paper 1

more papers are detail to atteched in a pdf files..................

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