Column Chromatography PDF

I am looking for the PDF file of Column Chromatography so please give me detail of it.

Column chromatography is suitable for the physical separation of gram quantities of material.

The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process.

Here I am attaching PDF file that have information about Column Chromatography

Objectives
1) To select a suitable solvent system for use in separation by thin‐layer chromatography.
2) To identify the compounds in a mixture by comparing their Rf values with reference
compounds.
3) To separate a two‐compound mixture using column chromatography and calculate
recovery percentages for each compound.

Columns:
The columns available in the department are simple glass tubes, varying in length and diameter.
They usually have a stopcock attached to control the solvent flow, and may have a fritted plate to support the adsorbent. Some columns may have a flask attached to the top of the column to act as a solvent reservoir.

Attached Files Available for Download

Column Chromatography.pdf (23.4 KB, 39 views)

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Answered By StudyChaCha Member

I am doing M.Sc Chemistry and searching for some details about Column Chromatography. Will you provide details of Column Chromatography in PDF and Column Chromatography procedure?

In chemistry, Column chromatography is a technique which is used to separate a single chemical compound from a mixture dissolved in a fluid.

It separates substances based on differential adsorption of compounds to the adsorbent as the compounds move through the column at different rates which allow them to get separated in fractions

Please find the below attached file for the Column Chromatography diagram:



Column Chromatography details






Procedure

Before starting with the Column Chromatography Experiment let us understand the different phases involved.

Mobile phase – This phase is made up of solvents and it performs the following functions:

It acts as a solvent – sample mixture can be introduced in the column.
It acts as a developing agent – helps in the separation of components in the sample to form bands.
It acts as an eluting agent – the components that are separated during the experiment are removed from the column
Some examples of solvents used as mobile phase based on their polarity are – ethanol, acetone, water, acetic acid, pyridine, etc.

Stationary phase – It is a solid material which should have good adsorption property and meet the conditions given below:

Shape and size of particle: Particles should have uniform shape and size in the range of 60 – 200μ in diameter.
Stability and inertness of particles: high mechanical stability and chemically inert. Also, no reaction with acids or bases or any other solvents used during the experiment.
It should be colourless, inexpensive and readily available.
Should allow free flow of mobile phase
It should be suitable for the separation of mixtures of various compounds.

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Answered By StudyChaCha Member