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Old January 5th, 2017, 03:27 PM
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Default PGMET Vector

Hi I would like to have the brief description for the pGEM®-T and pGEM®-T Easy Vector Systems as well as the advantages of its framewok?
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  #2  
Old January 5th, 2017, 03:52 PM
Educhamp
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Join Date: May 2011
Default Re: PGMET Vector

The pGEM®-T and pGEM®-T Easy Vector Systems are advantageous frameworks for the cloning of PCR items. The vectors are set up by cutting the pGEM®-5Zf(+) and pGEM®-T Easy Vectors, individually, with EcoR V and including a 3' terminal thymidine to both finishes. These single 3'- T overhangs at the inclusion site incredibly enhance the effectiveness of ligation of a PCR item into the plasmids by counteracting recircularization of the vector and giving a perfect shade to PCR items created by certain thermostable polymerases. These polymerases frequently include a solitary deoxyadenosine, in a layout autonomous mold, to the 3'- finishes of the opened up parts.

The high duplicate number pGEM®-T and pGEM®-T Easy Vectors contain T7 and SP6 RNA polymerase promoters flanking a different cloning district inside the alpha-peptide coding area of the catalyst beta-galactosidase. Insertional inactivation of the alpha-peptide permits recombinant clones to be straightforwardly distinguished by blue/white screening on pointer plates.

The pGEM®-T and pGEM®-T Easy Vector Systems incorporate a 2X Rapid Ligation Buffer for ligation of PCR items. Responses utilizing this support might be hatched for 1 hour at room temperature. The hatching time frame might be reached out to build the quantity of states after change.

Plasmid: pGEM-T Easy Vector

Source/Vendor: Promega

Alt Name: pGEM®-T Easy, pGEM-T Easy

Analyze: Sequence

Plasmid Type: Bacterial Expression

Expression Level: High

Cloning Method: Unknown

Size: 3015
5' Sequencing 1 Primer: T7, SP6, M13Fwd or M13Rev
Bacterial Resistance: Ampicillin

Notes:
The only difference between pGEM-T and pGEM-T Easy is in the multiple cloning site (MCS). The MCS of the pGEM-T Easy Vector contains sequences on either side of the insert that are recognized by the restriction enzymes Not I and EcoR I. This allows the insert DNA to be removed with a single restriction digest using either of these enzymes. Parental vector for TA cloning of PCR products.

Catalog Number: A1360

Stable: Transient

Constitutive: Constitutive

Viral/Non-Viral: Nonviral
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