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Old January 14th, 2014, 11:57 AM
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Join Date: Apr 2013

Give me information for MSC entrance examination organized by Pune university department of Microbiology ??

As you want to get Entrance Exam for MSC Microbiology so here I am giving you some entrance exam list:

The Indian Institute of Science (IISc) Entrance Exam
Indian Institute of Technology Entrance Exam
Mahatma Gandhi University: Faculty of Applied Science Entrance Exam
University of Calicut Microbiology Entrance Exam
University of Kerala: Faculty of Science Microbiology Entrance Exam
Aligarh Muslim University Microbiology Entrance Exam
Assam University Microbiology Entrance Exam
Banaras Hindu University Microbiology Entrance Exam
Goa University Microbiology Entrance Exam
Guru Nanak Dev University M.Sc. Microbiology Entrance Exam
Himachal Pradesh University Shimla - Microbiology Entrance Test
Lovely Professional University Microbiology Entrance Exam
Mahatma Gandhi University Microbiology Entrance Exam
Manipal Academy of Higher Education Microbiology Entrance Exam
Osmania University Microbiology Entrance Exam
University of Calcutta Microbiology Entrance Exam
University of Mumbai Microbiology Entrance Exam
University of Pune Microbiology Entrance Exam
University of Mysore Microbiology Entrance Exam

University of Mysore M.Sc. in Microbiology Entrance Exam Syllabus
Syllabus of M. Sc Entrance Examination for P .G. admission on Microbiology
General Microbiology: Milestones of the historical development of Microbiology- Discovery of
Microorganisms- Contributions of Scientists of Microbiology. Theory of spontaneous generation
and biogenesis. Different types of microscopes, their construction and working principles, Simple
and Compound microscope. Stereomicroscope. Principles, construction and mode of operation of
Scanning and transmission electron microscope, limitations. Preparation of specimens for electron
microscopic studies. Simple staining (positive and negative), differential staining (Gram’s staining
and acid – fast staining), structural staining (Capsule, Flagella, Cell wall and Endospore of
bacteria), nuclear staining, wet mounting method – staining of algae and fungi. Hanging drop
method. Principles and methods of sterilization.
The Microbial World: Groups of microorganisms: Viruses, Prokaryotes, and Eukaryotes. Study of
ultra structure of typical prokaryotic cell and eukaryotic cell, comparative account. General
Principles of classification and nomenclature of microorganisms (Haeckel’s three kingdom
classification and Whittaker’s five kingdom classification). General characteristics of viruses,
classification of viruses, isolation and identification of viruses. Study of structure and replication of
viruses: Bacteriophages T4 phage, Cyanophages. Phytophagenae TMV, Zoophagenae – Influenza
and HIV. Viroids and Prions. Ultra Structure of bacterial cell, classification of bacteria in brief as
per Bergey’s Manual of Systematic Bacteriology. Distribution, structure of typical algal cell.
General thallus structure and reproduction in algae and fungi.
Microbial Growth and Metabolism: Major nutritional types of microorganisms. Nutritional
requirements of microorganisms. Uptake of nutrients – passive and active transport. Microbial
Growth rate and generation time, growth curve – phases of growth and their significance.
Nomenclature, classification, properties of microbial enzymes mode and mechanism of enzyme
action, enzyme regulation, inhibition, cofactors and coenzymes. Photosynthetic microorganisms,
photosynthetic pigments and apparatus in prokaryotes and eukaryotes. Mechanism of
photosynthesis in bacteria. Anaerobic Respiration: breakdown of glucose to pyruvate – EMP, HMP
and ED pathways, Fermentation- conversion of pyruvate to ethanol and lactic acid. Aerobic
Respiration: formation of acetyl CoA from pyruvate, TCA Cycle, Electron transport and oxidative
phosphorylation, efficiency of aerobic and anaerobic respiration as energy yielding processes.
Symbiotic and asymbiotic nitrogen fixation, nodule formation, bacteroids, leghaemoglobin,
mechanism and biochemistry of N2 fixation, amino acid synthesis, proteolysis, nitrification and
denitrification. Biosynthesis of fatty acids, biosynthesis of triglyceriods and phospholipids,
degradation of fatty acids, oxidation of fatty acids.
Microbial Genetics: Chromosomes: Prokaryotic and eukaryotic organization. Recombination in
Bacteria:Transformation, Transduction and Conjugation process – F – factor, Sexduction,
Transposons. Extrachromosomal genetic elements and their importance. Structure and types of
DNA and RNA. Replication – mode and mechanism, replication in prokaryotic DNA – general
methodology, Gene – protein relationship: one gene – one enzyme and one gene – polypeptide
concept. Protien synthesis – transcription and translation, genetic code – features, triplet code,
Wobble hypothesis, nonsense codon, evolution of genetic code, regulation of gene expression in
prokaryotes. Nature and types of mutation. Mutagenic agents: physical and chemical mutagens,
damage and repair of DNA: Photoreactivation & SOS repair
Environmental microbiology: Microbes and atmosphere, Air spora of indoor and outdoor
environment, factors affecting air spora, significance of air-borne microbes, management of airborne
microbes. Microbiology of water: distribution of microorganisms in the aquatic environment.
Water pollution: Sources, water-borne diseases-viral (jaundice), bacterial (cholera) and protozoan,
(amoebic dysentry). Biological indicators of water pollution. Determination of sanitary
quality of water: SPC, Tests for coliform, MPN, IMVIC reactions, membrane filter technique.
Microbiology of waste water: Sources of waste water- domestic, agricultural and industrial,
physical, chemical and microbiological characteristics of waste water.
Waste water treatment: Single dwelling unit – septic tank: municipal waste water treatment-
Primary, secondary (trickling filter, activated sludge process, oxidation pond), Tertiary (reverse
osmosis, ion exchange method and dialysis) and reclamation of waste water. Solid waste recycling:
Anaerobic digestion process, Biogas and composting
Genetic engineering: Tools involved in genetic engineering: Modifying enzymes: Restriction
enzymes, ligases, methylases. Cloning vehicles – Naturally occurring plasmids, cloning plasmids
(PBR 322 and PUC 18). Viruses as cloning vehicles (DNA, M 13) and hybrid vectors (cosmid,
yac). DNA isolation, gel electrophoresis: Agarose gel-principle and method transformation
methods. DNA libraries: Brief account of Genomic and cDNA libraries – applications. Blottings:
Southern, Northern and Western. DNA sequencing: Brief account of Maxam and Gilbert’s
methods, Sanger’s method, automated sequencing method. Restriction fragment length
polymorphism. Molecular probes (Finger printing). Applications of genetic engineering in
Agriculture, Waste water management, Industries, Human health, Potential problems of genetic
engineering; social impact of genetic engineering.
Microorganisms in agriculture: Biofertilizers: Nitrogen fixing, Phosphate solubilizing and
cellulolytic microbes,mass production of bacterial inoculants (Rhizobium, Azotobacter,
Azospirillum, cyanobacteria) mode of applications, advantages and limitations. Biological control:
mechanism of antagonism, amensalism, competition predation and parasitism. Application of
biological control on field. Biopesticides: types – bacterial, viral, fungal and protozoan, mode of
action, target pests, use of transgenic plants. Rhizosphere microorganisms: Rhizosphere and
rhizoplane, interactions among micro organisms – neutralism, mutualism, commensalisms,
antagonism and parasitism Plant – microbe interaction – mycorrhizae. Plant pathology:
classification of plant diseases, principles of infection and spread of diseases in general.
Food Microbiology: Food as a substrate for growth of microorganism’s .Microbial spoilage of
food and food preservation techniques. Microbial spoilage of food – Fruits, vegetables, meat,
poultry, canned foods. Important methods of food preservations. Physical method- High
temperature, Canning, Low temperature, Drying, radiation and Chemical preservatives. Mycotoxin
with reference to Aflatoxin, Staphylococcal intoxication, Botulism, Salmonellosis. Food safety and
quality control. A brief account of food laws and standards. Sources of milk contamination.
Methods to detect microbial spoilage by SPC, Reductase test, Biochemical changes of milk. Starter
culture and its role. Fermented dairy products. Acidophilus milk, Yogurt, Butter milk, Srikhand.
Types of cheese, Preparation of cheese. Preservation of milk and milk products – Pasteurization,
Industrial Microbiology: Microorganisms of industrial importance. Types of stock culture. Strain
improvement. Fermentation media : A brief account of Production medium, Inoculum medium,
Raw materials – Molasses and types, corn steep liquor, sulphite waste liquor and whey, Buffers,
Precursors, Inhibitors, and Antifoam agents.
Design of typical fermentors, Devices for aeration, agitation. Fermentation process-Surface,
Submerged and Solid state fermentation: Types-Batch and Continuous fermentation. Down stream
processing – Precipitation, filtration, centrifugation, distillation, cell disruption, solvent recovery,
drying, crystallization Industrial production and its uses of the following: Ethyl alcohol, Wine
Antibiotics – Penicillin. Organic acid – Lactic acid. Enzymes – Amylase, Protease, Single Cell
Protein, Mushroom cultivation, Role of microorganisms for production and recovery of minerals
and petroleum.
Immunology and Medical microbiology: introduction to immune system: Types of immunity-
Innate and Adaptive Immunity. Antibody mediated immunity and Cell mediated immunity. Cells
and tissues of immune system – Structure and role of primary lymphoid organs (bone marrow,
thymus), secondary lymphoid organs (spleen, lymph nodes and tonsils) B&T lymphocytes,
phagocytes, killer cells, NK cells.
Antigens – nature and types. Antibodies – Structure of lgG. Classes of antibodies and their
functional diversity, Human blood types and Rh factors, Antigen-antibody reactions-salient
features. Agglutination reaction. Immunotechniques – RIA, ELISA. Complement system.
Immunoprophylaxis – Vaccine-Types – Killed, Live attenuated (bacterial and viral) and Toxoid.
Pathogenesis, clinical symptoms, laboratory diagnosis, epidemiology, prophylaxis and treatment of
the following -Bacterial diseases-Tuberculosis, Cholera, Typhoid, Syphilis. Viral diseases –
Hepatitis, Poliomyelitis, AIDS. Fungal diseases-Candidacies, Dermatomycosis (Tinea – ringworm
infection). Protozoan diseases – Malaria, Trichomoniasis.
Chemotherapy: General characteristics and types of antibiotics.

Last edited by Aakashd; October 12th, 2019 at 05:11 PM.
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Old January 14th, 2014, 12:38 PM
Super Moderator
Join Date: Jun 2013
Default Re: Entrance Exam for MSC Microbiology

Here I am giving you information for MSC entrance examination organized by Pune university department of Microbiology

To participate in the entrance exam you have to pass B.Sc. Microbiology or B.Sc. with Microbiology from any well recognized university ..

Important tentative dates :
Issue of forms : April 2014
Last date for submission : may 2014
Date of entrance exam : june 2014

Exam pattern :
Nature of Questions Objective only
Number of Questions 50
Marks per question Two
Total Marks 100
Passing criteria Not Applicable
Negative Marking -1 for each wrong answered question.
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