Baba Saheb Bhim Rao Ambedkar University Lucknow
Re: Baba Saheb Bhim Rao Ambedkar University Lucknow
As you are looking for the course structure of M.Sc Biotechnology as well as detailed syllabus of its first Semester offered at Baba Saheb Bhim Rao Ambedkar University, Lucknow so here I am sharing with you same.
Course structure of M.Sc Biotechnology:
Semester - I
MBT - 101 Cell Biology
MBT - 102 Biomolecules
MBT - 103 Microbial Physiology & Genetics
MBT - 104 Biophysical Tools & Techniques
MBT - 105 Laboratory Course-1
Semester - II
MBT - 201 Molecular Biology
MBT - 202 Enzymology
MBT - 203 Immunology
MBT - 204 Bioprocess Engg. & Technology
MBT - 205 Laboratory Course -II
Semester - III
MBT - 301 Animal Cell Science & Technology
MBT - 302 Environmental Biotechnology
MBT - 303 Plant Biotechnology
MBT - 304 Genetic Engineering
MBT - 305 Laboratory Course –II
Semester - IV
MBT - 401 Seminar
MBT - 402 Research Project
Semester I Detailed syllabus:
MBT – 101 MBT-101 CELL BIOLOGY (Max. Marks 100)
Structure of prokaryotic and eukaryotic cell – Subcellular fractionation and criteria of functional integrity.
The structure of cellular organelles: Plasma membrane, cell wall, cytoskeleton their structural organization and extra cellular matrix .Mitochondria, chloroplast, ribosomes , lysosome , nucleus, and other organelles and their organization.
Biological membranes- Physicochemical properties of cell membranes and their structural constitution. Transport of nutrients across the membranes –simple, passive, facilitated diffusion, Protein targeting and sorting- Post transitional import of proteins to mitochondria lysosomes, nucleus, secretary vesicles, chloroplast and peroxisomes.
Cellular responses to environmental signals in bacteria, plants and animals-mechanism of signal transduction. Cell cycle – molecular events and regulation.
Cellular basis of differentiation and development – cell division, gametogenesis and fertilization, differential gene activity and cell differentiation, morphogenetic determinants in egg cytoplasm, genetic regulation of early embryonic development in Drosophilla, homeotic genes.
MBT – 102 MBT-102 BIOMOLECULES (Max. Marks 100)
Classification & properties of mono-, di-, oligo and poly- saccharides. Structural features and compositional analysis of polysaccharides. Biological importance of glucose, fructose, maltose, sucrose, lactose, starch, glycogen, lignin, cellulose, peptidoglycan and glycoproteins.
Classification, structure, properties and functions of lipids. Biological importance of choline, lecithine, lipoproteins, chylomicrons , VLDL, LDL, HDL.
Classification and physico-chemical properties of amino acids and proteins. Isolation, purification and criteria of purity of proteins. Definition, characteristics and determination of primary, secondary, tertiary and quaternary structures.
Structure, properties and functions of nucleic acids. Sequencing of DNA, DNA Polymorphism, Tm and its relation to GC content, Cot value.
Classification and properties of porphyrins, metalloproteins. Nature, synthesis and physiological significance of bile pigments.
MBT – 103 MICROBIAL PHYSIOLOGY & GENETICS (Max. Marks 100)
History, development and scope of Microbiology, Prokaryotic cells: structure and function, cell envelope (outer and inner membrane, surface polysaccharides, peptidoglycan, periplasmic space); flagella, pili, cell inclusions i.e, endospores, gas vesicles.
Prokaryotic diversity, Systematics and Taxonomy; Modern approaches to bacterial taxonomy, polyphasic classification; Ribosomal DNA sequencing, General characteristics of primary domains and of taxonomics groups belonging to Bacteria, Archea and Ekarya. Nomenclature and outline of bacterial classification as per Bergey’s manual. Bacterial genetic system: Transformation, conjugation, Transduction, Recombination, Plasmids and Transposon . Bacterial genetic map with reference to E.coli.
Methods in Microbiology: Theory and practice of sterilization, pure culture techniques; construction of culture media; enrichment of culture techniques, isolation and culture of aerobic and anaerobic bacteria; culture collection, preservation and maintenance of microbial cultures. Microbial growth: mathematical expression of growth, growth curve, measurement of growth and growth yields; synchronous growth; continuous culture; environmental factors affecting growth: temperature, acidity, alkalinity, water availability and oxygen.
Metabolic Diversity among Microorganisms .principles of microbial Nutrition; Nutrition types and modes of nutrition in bacteria .Bacterial photosynthesis; structural and functional properties of pigments. Oxygenic and anoxygenic photosynthesis and photodynamic death. Chemolithotherapy; Hydrogen –iron –nitrite- oxidising bacteria; nitrate and sulphate reduction; Methanogenesis and acetogenesis; Nitrogen metabolism; nitrogen fixation; hydrogen transformation.
Host-parasite relationships- Normal micro-flora of human body, entry of pathogens into the host: Colonization and factors predisposing to infections;: Types of toxins (Exo-, Endo-, Entero-) and their structure and mode of actions, virulence and pathogenesis .Microbial diseases: Epidemiology of infectious disease with reference to tuberculosis, cholera, sexually transmitted diseases (AIDS), diseases transmitted by animals (rabies), insects (malaria) food (Salmonella ) and pathogenic fungi.
Chemotherapy / Antibiotics: Antimicrobial agents ; Antibiotics: Penicillins and Cephalosporins; Broad spectrum antibiotics , sulfa drugs ; antifungal antibiotics; mode of action ; molecular mechanism of drug resistance of viruses :General properties , structure and classification of viruses based on their genomes ; Bacterial(phage Lambda ), Plant (CaMV) , Animal (Hepatitis, retroviruses) viruses, virioids and prions . Viruses and Their Genetic system. Phage I and its life cycle; RNA phages; RNA viruses; Retroviruses.
MBT – 104 BIOPHYSICAL TOOLS & TECHNIQUES (Max. Marks 100)
Chemical foundations of biology: Buffers, pH and pK of acids and bases, Weak and covalent interactions among the biomolecules. Hydrodynamic methods - Centrifugation- basic principles of sedimentation, types of analytical and preparative centrifugation. Viscosity, Osmosis and surface tension in molecular weight determination.
Chromatography- Principles and types chromatography (Ascending and Descending; column chromatography- adsorption, ion-exchange, affinity, GLC,HPLC). Electrophoresis principles and types of electrophoresis (PAGE, PFGE, Capillary, 2D electrophoresis; Microchip electrophoresis).
Spectroscopy: UV-Vis, IR, FT-IR and mass spectroscopy. Microscopy: principlesof –Phase contrast and florescence microscopy, fixation and staining, SEM and flow cytometry.
NMR: basic principles; chemical shift; Use of NMR in studying protein structure and X-ray diffraction. Radioisotope techniques: Application of radioisotopes in biology; autoradiography and radiation dosimetry. Nanotechnology- Nanoparticles; their application in medicine and biology.
Bioinformatics: Introduction, Information sources for nucleotide and protein sequences. BLAST, multiple sequence alignment, Phylogenetic analysis. Computer aided drug designing, computational techniques in structural analysis and in detection of pathogens. Statistical analysis of biochemical data: Standard deviation, mean, mode and least square analysis. Students T test and Chi square test.
MBT – 105 LABORATORY COURSE (Max. Marks 100)
Titration of aminoacids and determination of pK.
Model building using space filling / ball and stick models.
Identification of aminoacids , sugars and lipids by TLC and/ or colour reactions.
Isolation and quantitation of Nucleic acids.
Quantization of properties, sugars and cholesterol by different methods.
Determination of iodine, saponification and acid no. of lipid/ oil samples .
Separation of proteins by gel filtration and ion exchange chromatography .
Microscopy: Bright field, phase contrast and fluorescence microscopy.
Microtomy and Histochemical techniques.
Mitosis and meiosis.
Preparation of liquid and solid media.
Pure culture isolation and maintenance of organisms from soil and water by plating, streaking and serial dilution methods.
Slants and Stab cultures for preservation of microorganism.
Growth curve, measurement of bacterial population by turbidometry and serial dilution methods, Effect of temperature, pH and sores on growth.
Microscopic examination of bacteria, yeast and moulds and study of organisms by Gram stain, Acid fast stain and staining for spores.
Analysis of water for potability and determination of MPN.
Biochemical characterization of selected microbes.
One step growth curve of coliphage.
Assay of antibiotic and demonstration of antibiotic resistence.
CO2 fixation by photosynthesic microbes.
Electrophoresis of proteins-Native and SDS-AGE.
N- and C-terminal analysis of proteins.
Separation techniques (HPLC , GLC ,FPLC)
Hydrodynamic properties- Measurement and applicaton.
For more detail you can contact at:
Babasaheb Bhimrao Ambedkar University
Rai Bareilly Road
Phone : 91-522-2440822
Fax : 91-522-2440821,2441888
E-mail : email@example.com
Answered By StudyChaCha Member
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